❞ كتاب Mass Spectrometry of Proteins and Peptides-Humana Press ❝  ⏤ John R. Chapman

❞ كتاب Mass Spectrometry of Proteins and Peptides-Humana Press ❝ ⏤ John R. Chapman

نبذه عن الكتاب:

Recent developments in technology and instrumentation have made mass
spectrometry the method of choice for the identification of gel-separated proteins using rapidly growing sequence databases (1). Proteins with a full-length
sequence present in a database can be identified with high certainty and high
throughput using the accurate masses obtained by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry peptide mapping (2). Simple protein mixtures can also be deciphered by MALDI peptide mapping (3) and the
entire identification process, starting from in-gel digestion (4) and finishing
with acquisition of mass spectra and database search, can be automated (5).
Only 1–3% of a total digest are consumed for MALDI analysis even if the
protein of interest is present on a gel in a subpicomole amount. If no conclusive
identification is achieved by MALDI peptide mapping, the remaining protein
digest can be analyzed by nanoelectrospray tandem mass spectrometry (Nano
ESI-MS/MS) (6). Nano ESI-MS/MS produces data that allow highly specific
database searches so that proteins that are only partially present in a database,
or relevant clones in an EST database, can be identified (7). It is important to
point out that there is no need to determine the complete sequence of peptides
in order to search a database—a short sequence stretch consisting of three to
four amino acid residues provides enough search specificity when combined
with the mass of the intact peptide and the masses of corresponding fragment John R. Chapman - ❰ له مجموعة من الإنجازات والمؤلفات أبرزها ❞ Mass Spectrometry of Proteins and Peptides-Humana Press ❝ ❱
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نبذة عن الكتاب:
Mass Spectrometry of Proteins and Peptides-Humana Press

2000م - 1446هـ
نبذه عن الكتاب:

Recent developments in technology and instrumentation have made mass
spectrometry the method of choice for the identification of gel-separated proteins using rapidly growing sequence databases (1). Proteins with a full-length
sequence present in a database can be identified with high certainty and high
throughput using the accurate masses obtained by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry peptide mapping (2). Simple protein mixtures can also be deciphered by MALDI peptide mapping (3) and the
entire identification process, starting from in-gel digestion (4) and finishing
with acquisition of mass spectra and database search, can be automated (5).
Only 1–3% of a total digest are consumed for MALDI analysis even if the
protein of interest is present on a gel in a subpicomole amount. If no conclusive
identification is achieved by MALDI peptide mapping, the remaining protein
digest can be analyzed by nanoelectrospray tandem mass spectrometry (Nano
ESI-MS/MS) (6). Nano ESI-MS/MS produces data that allow highly specific
database searches so that proteins that are only partially present in a database,
or relevant clones in an EST database, can be identified (7). It is important to
point out that there is no need to determine the complete sequence of peptides
in order to search a database—a short sequence stretch consisting of three to
four amino acid residues provides enough search specificity when combined
with the mass of the intact peptide and the masses of corresponding fragment
.
المزيد..

تعليقات القرّاء:

Biologically

Biology is a natural science that is concerned with the study of life, its various forms and its function, how these organisms interact with each other and with the surrounding environment. The word biology in Greek is made up of two words: bio (βίος) meaning life. And loggia (-λογία) means science or study. Biology: the similarity of vegetation and animal cover on the edges of the African and American states, and the existence of the same fossil.


Branches of biology
Biology is an ancient science thousands of years old and modern biology began in the nineteenth century. This science has multiple branches. Among them are:

Anatomy
Botany
Biochemia
Biogeography
Biofisia
Cytology or cell science
Ecology or environmental science

 

 

نبذه عن الكتاب:

Recent developments in technology and instrumentation have made mass
spectrometry the method of choice for the identification of gel-separated proteins using rapidly growing sequence databases (1). Proteins with a full-length
sequence present in a database can be identified with high certainty and high
throughput using the accurate masses obtained by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry peptide mapping (2). Simple protein mixtures can also be deciphered by MALDI peptide mapping (3) and the
entire identification process, starting from in-gel digestion (4) and finishing
with acquisition of mass spectra and database search, can be automated (5).
Only 1–3% of a total digest are consumed for MALDI analysis even if the
protein of interest is present on a gel in a subpicomole amount. If no conclusive
identification is achieved by MALDI peptide mapping, the remaining protein
digest can be analyzed by nanoelectrospray tandem mass spectrometry (Nano
ESI-MS/MS) (6). Nano ESI-MS/MS produces data that allow highly specific
database searches so that proteins that are only partially present in a database,
or relevant clones in an EST database, can be identified (7). It is important to
point out that there is no need to determine the complete sequence of peptides
in order to search a database—a short sequence stretch consisting of three to
four amino acid residues provides enough search specificity when combined
with the mass of the intact peptide and the masses of corresponding fragment

Biology
Human biology
Who is the founder of biology?
The importance of biology
Areas of work in the field of biology
Theories of biology
Research on biology for the first grade of secondary school
Human biology

 



سنة النشر : 2000م / 1421هـ .
حجم الكتاب عند التحميل : 3.045 .
نوع الكتاب : pdf.
عداد القراءة: عدد قراءة Mass Spectrometry of Proteins and Peptides-Humana Press

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المؤلف:
John R. Chapman -

كتب John R. Chapman ❰ له مجموعة من الإنجازات والمؤلفات أبرزها ❞ Mass Spectrometry of Proteins and Peptides-Humana Press ❝ ❱. المزيد..

كتب John R. Chapman