❞ كتاب Extracellular Matrix Protocols ❝  ⏤ Charles H. Streuli
Michael E. Grant

❞ كتاب Extracellular Matrix Protocols ❝ ⏤ Charles H. Streuli Michael E. Grant

نبذه عن الكتاب:

Semipermeabilized Cells
to Study Procollagen Assembly
Richard R. Wilson and Neil J. Bulleid
1. Introduction
This chapter will describe the preparation and use of a semipermeabilized
(SP) cell system that reconstitutes the initial stages in the assembly and modification of proteins entering the secretory pathway (1). The procedure involves
treating cells grown in culture with the detergent digitonin and isolating the
cells free from their cytosolic component (2). The expression of proteins in an
SP cell system allows protein assembly to be studied in an environment that
more closely resembles that of the intact cell. As this is an in vitro system, the
individual components can be manipulated easily, providing a means by which
cellular processes can be studied under a variety of conditions. In addition,
membrane-permeable chemical crosslinking reagents can be added in order to
facilitate the study of interaction between proteins within the endoplasmic
reticulum (ER) lumen (1,3). Furthermore, as the ER remains morphologically
intact, the spatial localization of folding and transport processes within the
reticular network may also be examined
Charles H. Streuli
Michael E. Grant - ❰ له مجموعة من المؤلفات أبرزها ❞ Extracellular Matrix Protocols ❝ ❱
من Biology Books علم الأحياء - مكتبة الكتب العلمية.

نبذة عن الكتاب:
Extracellular Matrix Protocols

2000م - 1444هـ
نبذه عن الكتاب:

Semipermeabilized Cells
to Study Procollagen Assembly
Richard R. Wilson and Neil J. Bulleid
1. Introduction
This chapter will describe the preparation and use of a semipermeabilized
(SP) cell system that reconstitutes the initial stages in the assembly and modification of proteins entering the secretory pathway (1). The procedure involves
treating cells grown in culture with the detergent digitonin and isolating the
cells free from their cytosolic component (2). The expression of proteins in an
SP cell system allows protein assembly to be studied in an environment that
more closely resembles that of the intact cell. As this is an in vitro system, the
individual components can be manipulated easily, providing a means by which
cellular processes can be studied under a variety of conditions. In addition,
membrane-permeable chemical crosslinking reagents can be added in order to
facilitate the study of interaction between proteins within the endoplasmic
reticulum (ER) lumen (1,3). Furthermore, as the ER remains morphologically
intact, the spatial localization of folding and transport processes within the
reticular network may also be examined .
المزيد..

تعليقات القرّاء:

Biologically

Biology is a natural science that is concerned with the study of life, its various forms and its function, how these organisms interact with each other and with the surrounding environment. The word biology in Greek is made up of two words: bio (βίος) meaning life. And loggia (-λογία) means science or study. Biology: the similarity of vegetation and animal cover on the edges of the African and American states, and the existence of the same fossil.


Branches of biology
Biology is an ancient science thousands of years old and modern biology began in the nineteenth century. This science has multiple branches. Among them are:

Anatomy
Botany
Biochemia
Biogeography
Biofisia
Cytology or cell science
Ecology or environmental science

 

 

نبذه عن الكتاب:

Semipermeabilized Cells
to Study Procollagen Assembly
Richard R. Wilson and Neil J. Bulleid
1. Introduction
This chapter will describe the preparation and use of a semipermeabilized
(SP) cell system that reconstitutes the initial stages in the assembly and modification of proteins entering the secretory pathway (1). The procedure involves
treating cells grown in culture with the detergent digitonin and isolating the
cells free from their cytosolic component (2). The expression of proteins in an
SP cell system allows protein assembly to be studied in an environment that
more closely resembles that of the intact cell. As this is an in vitro system, the
individual components can be manipulated easily, providing a means by which
cellular processes can be studied under a variety of conditions. In addition,
membrane-permeable chemical crosslinking reagents can be added in order to
facilitate the study of interaction between proteins within the endoplasmic
reticulum (ER) lumen (1,3). Furthermore, as the ER remains morphologically
intact, the spatial localization of folding and transport processes within the
reticular network may also be examined

Biology
Human biology
Who is the founder of biology?
The importance of biology
Areas of work in the field of biology
Theories of biology
Research on biology for the first grade of secondary school
Human biology

 



سنة النشر : 2000م / 1421هـ .
حجم الكتاب عند التحميل : 2.285 .
نوع الكتاب : pdf.
عداد القراءة: عدد قراءة Extracellular Matrix Protocols

اذا اعجبك الكتاب فضلاً اضغط على أعجبني
و يمكنك تحميله من هنا:

تحميل Extracellular Matrix Protocols
شكرًا لمساهمتكم

شكراً لمساهمتكم معنا في الإرتقاء بمستوى المكتبة ، يمكنكم االتبليغ عن اخطاء او سوء اختيار للكتب وتصنيفها ومحتواها ، أو كتاب يُمنع نشره ، او محمي بحقوق طبع ونشر ، فضلاً قم بالتبليغ عن الكتاب المُخالف:

برنامج تشغيل ملفات pdfقبل تحميل الكتاب ..
يجب ان يتوفر لديكم برنامج تشغيل وقراءة ملفات pdf
يمكن تحميلة من هنا 'http://get.adobe.com/reader/'

المؤلف:
Charles H. Streuli Michael E. Grant -

كتب Charles H. Streuli
Michael E. Grant ❰ له مجموعة من المؤلفات أبرزها ❞ Extracellular Matrix Protocols ❝ ❱. المزيد..

كتب Charles H. Streuli Michael E. Grant