❞ كتاب Protein Signaling_ Methods and Protocols ❝  ⏤ Alan V. Smrcka

❞ كتاب Protein Signaling_ Methods and Protocols ❝ ⏤ Alan V. Smrcka

نبذه عن الكتاب:

Purification of Recombinant G Protein α Subunits
from Escherichia coli
Wendy K. Greentree and Maurine E. Linder
Summary
The purification of recombinant G protein a subunits expressed in Escherichia coli (E. coli) is a convenient and inexpensive method to obtain homogeneous preparations of protein for biochemical and biophysical analyses.
Wild-type and mutant forms of Gα are easily produced for analysis of their
intrinsic biochemical properties, as well as for reconstitution with receptors,
effectors, regulators, and G protein βγ subunits. Methods are described for the
expression of Gi
α and Gsα proteins in E. coli. Protocols are provided for the
purification of untagged G protein a subunits using conventional chromatography and histidine (His)-tagged subunits using metal chelate chromatography.
Modification of Gα with myristate can be recapitulated in E. coli by expressing N-myristoyltransferase (NMT) with its G protein substrate. Protocols for
the production and purification of myristoylated Gα are presented.
Key Words: G protein; α subunit; signal transduction; protein purification; affinity chromatography; GTPase; membrane protein; myristoylation;
N-myristoyltransferase. Alan V. Smrcka - ❰ له مجموعة من المؤلفات أبرزها ❞ Protein Signaling_ Methods and Protocols ❝ ❱
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نبذة عن الكتاب:
Protein Signaling_ Methods and Protocols

2010م - 1444هـ
نبذه عن الكتاب:

Purification of Recombinant G Protein α Subunits
from Escherichia coli
Wendy K. Greentree and Maurine E. Linder
Summary
The purification of recombinant G protein a subunits expressed in Escherichia coli (E. coli) is a convenient and inexpensive method to obtain homogeneous preparations of protein for biochemical and biophysical analyses.
Wild-type and mutant forms of Gα are easily produced for analysis of their
intrinsic biochemical properties, as well as for reconstitution with receptors,
effectors, regulators, and G protein βγ subunits. Methods are described for the
expression of Gi
α and Gsα proteins in E. coli. Protocols are provided for the
purification of untagged G protein a subunits using conventional chromatography and histidine (His)-tagged subunits using metal chelate chromatography.
Modification of Gα with myristate can be recapitulated in E. coli by expressing N-myristoyltransferase (NMT) with its G protein substrate. Protocols for
the production and purification of myristoylated Gα are presented.
Key Words: G protein; α subunit; signal transduction; protein purification; affinity chromatography; GTPase; membrane protein; myristoylation;
N-myristoyltransferase.
.
المزيد..

تعليقات القرّاء:

Biologically

Biology is a natural science that is concerned with the study of life, its various forms and its function, how these organisms interact with each other and with the surrounding environment. The word biology in Greek is made up of two words: bio (βίος) meaning life. And loggia (-λογία) means science or study. Biology: the similarity of vegetation and animal cover on the edges of the African and American states, and the existence of the same fossil.


Branches of biology
Biology is an ancient science thousands of years old and modern biology began in the nineteenth century. This science has multiple branches. Among them are:

Anatomy
Botany
Biochemia
Biogeography
Biofisia
Cytology or cell science
Ecology or environmental science

 

 

نبذه عن الكتاب:

Purification of Recombinant G Protein α Subunits
from Escherichia coli
Wendy K. Greentree and Maurine E. Linder
Summary
The purification of recombinant G protein a subunits expressed in Escherichia coli (E. coli) is a convenient and inexpensive method to obtain homogeneous preparations of protein for biochemical and biophysical analyses.
Wild-type and mutant forms of Gα are easily produced for analysis of their
intrinsic biochemical properties, as well as for reconstitution with receptors,
effectors, regulators, and G protein βγ subunits. Methods are described for the
expression of Gi
α and Gsα proteins in E. coli. Protocols are provided for the
purification of untagged G protein a subunits using conventional chromatography and histidine (His)-tagged subunits using metal chelate chromatography.
Modification of Gα with myristate can be recapitulated in E. coli by expressing N-myristoyltransferase (NMT) with its G protein substrate. Protocols for
the production and purification of myristoylated Gα are presented.
Key Words: G protein; α subunit; signal transduction; protein purification; affinity chromatography; GTPase; membrane protein; myristoylation;
N-myristoyltransferase.

Biology
Human biology
Who is the founder of biology?
The importance of biology
Areas of work in the field of biology
Theories of biology
Research on biology for the first grade of secondary school
Human biology

 



سنة النشر : 2010م / 1431هـ .
حجم الكتاب عند التحميل : 1.997 .
نوع الكتاب : pdf.
عداد القراءة: عدد قراءة Protein Signaling_ Methods and Protocols

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كتب Alan V. Smrcka ❰ له مجموعة من المؤلفات أبرزها ❞ Protein Signaling_ Methods and Protocols ❝ ❱. المزيد..

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