❞ كتاب Peptide Nucleic Acids Methods and Protocols ❝ ⏤ Peter E. Nielsen

نبذه عن الكتاب:

PNA Technology
Peter E. Nielsen
1. Introduction
Peptide nucleic acids (PNA) were originally conceived and
designed as sequence-specific DNA binding reagents targeting the
DNA major groove in analogy to triplex-forming oligonucleotides.
However, instead of the sugar-phosphate backbone of oligonucleotides PNA was designed with a pseudopeptide backbone (1). Once
synthesized, it was apparent that PNA oligomers based on the
aminoethylglycin backbone with acetyl linkers to the nucleobases
(see Fig. 1) are extremely good structural mimics of DNA (or
RNA), being able to form very stable duplex structures with
Watson-Crick complementary DNA, RNA (or PNA) oligomers (2–
4). It also quickly became clear that triplexes formed between one
homopurine DNA (or RNA) strand and two sequence complementary PNA strands are extraordinarily stable. Furthermore, this stability is the reason why homopyrimidine PNA oligomers when
binding complementary targets in double-stranded DNA do not do
so by conventional (PNA-DNA2) triplex formation, but rather prefer to form a triplex-invasion complex in which the DNA duplex is
invaded by an internal PNA2-DNA triplex (see Fig. 2) (5,6). This
type of binding is restricted to homopurine/homopyrimidine DNA
targets in full analogy to dsDNA targeting by triplex forming oligo
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نبذة عن الكتاب:

Peptide Nucleic Acids Methods and Protocols

2002م - 1446هـ
نبذه عن الكتاب:

PNA Technology
Peter E. Nielsen
1. Introduction
Peptide nucleic acids (PNA) were originally conceived and
designed as sequence-specific DNA binding reagents targeting the
DNA major groove in analogy to triplex-forming oligonucleotides.
However, instead of the sugar-phosphate backbone of oligonucleotides PNA was designed with a pseudopeptide backbone (1). Once
synthesized, it was apparent that PNA oligomers based on the
aminoethylglycin backbone with acetyl linkers to the nucleobases
(see Fig. 1) are extremely good structural mimics of DNA (or
RNA), being able to form very stable duplex structures with
Watson-Crick complementary DNA, RNA (or PNA) oligomers (2–
4). It also quickly became clear that triplexes formed between one
homopurine DNA (or RNA) strand and two sequence complementary PNA strands are extraordinarily stable. Furthermore, this stability is the reason why homopyrimidine PNA oligomers when
binding complementary targets in double-stranded DNA do not do
so by conventional (PNA-DNA2) triplex formation, but rather prefer to form a triplex-invasion complex in which the DNA duplex is
invaded by an internal PNA2-DNA triplex (see Fig. 2) (5,6). This
type of binding is restricted to homopurine/homopyrimidine DNA
targets in full analogy to dsDNA targeting by triplex forming oligo . المزيد..

تعليقات القرّاء:

Biologically

Biology is a natural science that is concerned with the study of life, its various forms and its function, how these organisms interact with each other and with the surrounding environment. The word biology in Greek is made up of two words: bio (βίος) meaning life. And loggia (-λογία) means science or study. Biology: the similarity of vegetation and animal cover on the edges of the African and American states, and the existence of the same fossil.

Branches of biology
Biology is an ancient science thousands of years old and modern biology began in the nineteenth century. This science has multiple branches. Among them are:

Anatomy
Botany
Biochemia
Biogeography
Biofisia
Cytology or cell science
Ecology or environmental science

نبذه عن الكتاب:

PNA Technology
Peter E. Nielsen
1. Introduction
Peptide nucleic acids (PNA) were originally conceived and
designed as sequence-specific DNA binding reagents targeting the
DNA major groove in analogy to triplex-forming oligonucleotides.
However, instead of the sugar-phosphate backbone of oligonucleotides PNA was designed with a pseudopeptide backbone (1). Once
synthesized, it was apparent that PNA oligomers based on the
aminoethylglycin backbone with acetyl linkers to the nucleobases
(see Fig. 1) are extremely good structural mimics of DNA (or
RNA), being able to form very stable duplex structures with
Watson-Crick complementary DNA, RNA (or PNA) oligomers (2–
4). It also quickly became clear that triplexes formed between one
homopurine DNA (or RNA) strand and two sequence complementary PNA strands are extraordinarily stable. Furthermore, this stability is the reason why homopyrimidine PNA oligomers when
binding complementary targets in double-stranded DNA do not do
so by conventional (PNA-DNA2) triplex formation, but rather prefer to form a triplex-invasion complex in which the DNA duplex is
invaded by an internal PNA2-DNA triplex (see Fig. 2) (5,6). This
type of binding is restricted to homopurine/homopyrimidine DNA
targets in full analogy to dsDNA targeting by triplex forming oligo

Biology
Human biology
Who is the founder of biology?
The importance of biology
Areas of work in the field of biology
Theories of biology
Research on biology for the first grade of secondary school
Human biology

سنة النشر : 2002م / 1423هـ .
حجم الكتاب عند التحميل : 2.079 .
نوع الكتاب : pdf.
عداد القراءة: عدد قراءة Peptide Nucleic Acids Methods and Protocols

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Peter E. Nielsen -

❰ له مجموعة من الإنجازات والمؤلفات أبرزها ❞ Peptide Nucleic Acids Methods and Protocols ❝ ❱. المزيد..

كتب Peter E. Nielsen